Table V.
Primers and conditions used for semiquantitative RT-PCR
| Target Gene | EST/TC No. | GenBank Accession No. of EST | Forward Primer (5′–3′) | Reverse Primer (5′–3′) | Product Size | Annealing Tm/Cycles |
|---|---|---|---|---|---|---|
| bp | ||||||
| Peroxidase | TC397 | CV542921 | CCA ACC AAA CAC TTG CCA ATG | GAG TAG TAG GCC TTG TCG AAT | 313 | 58°C/20 |
| Glycolipid transfer protein-like | TC1903 | CV543709 | GTT GTT CTC AGT CTG CGA TCA | TAT TGG AGT GGA TGG CAA CGA | 751 | 60°C/25 |
| Translationally controlled tumor protein | TC63 | CV542788 | CGC TCC GCA CCA GTT ATC A | GGA TCA GTG GCA CCG TCC TTG | 528 | 60°C/25 |
| No BLAST hit | RTS_103_E03 | CV541966 | GGC TTC AAA ATC CTC ACG C | GCT TGT TGC TAT CTC AAA CGC | 214 | 60°C/20 |
| UDP-Glc-6-dehydrogenase | TC1804 | CV541174 | GGC TTT ATG TTC TTC TAT GTT | TGA AAC CCT CAA ATA TTA CTC | 138 | 52°C/25 |
| Cytosolic aldehyde dehydrogenase | TC733 | CV542619 | CCG TGT GCT CGT TCA AG | ATC TCC TCC TGC ACT ATT CTC | 282 | 52°C/25 |
| Senescence-related dihydroorotate dehydrogenase | RTS_109_B06 | EH792671 | ATA GCA CTT GGG AAG GTG ATG | GTCTTTGTCAGATTG CAAGCC | 359 | 55°C/25 |
| ACC oxidase | TC280 | CV542243 | TGG CAC CAA AGT TAG CAA CTA | ATT CTG GTG CCA TCC GTT TGA | 270 | 57°C/25 |
| Hypothetical protein | TC1992 | CV542243 | AAC AGT CAA AAG TAT CAC ACG | GAT TCA GGG TTT CAG TAT AGG | 219 | 55°C/25 |
| Isocitrate dehydrogenase (NADP) | TC1864 | CV541923 | CCC TGA CGG AAA GAC TAT TGA | ACC TTT ACT TTC TGC GGT GCC | 593 | 60°C/20 |
| S-adenosyl Met decarboxylase | TC83 | CV543847 | TAC TTG GGA CTG TTG TTA TCA | TAA ACT CAC GGG TAT AGC TTG | 1,589 | 60°C/20 |
| Ribosomal protein 40 S | TC1613 | CV543229 | TTT CTA GGG TTT CGC TCG GTA | TAA ACA GAA ACA AAC GCC ATA | 600 | 60°C/25 |
| Transporter-like protein | TC2875 | CV543019 | TGC TTC TTC GCC ACA ACT CAA | CCT TCC AAG ATG TAG CCG TCA | 476 | 60°C/25 |
| Trehalose-6-P synthase | RTS_114_F03 | CV542030 | TTT CTA AGA ATG AAG CGA GAT | GAT CCA TCA GTT GTT TCG GTA | 116 | 52°C/25 |
| Phosphoribosylanthranilate transferase | RTS_139_D07 | CV543777 | TGG CCA TGC AGA TTG TG | GGA TTA ACA TTA TGC GAT GAA | 144 | 52°C/25 |
| Caffeine-induced death protein 1 | RTS_143_D10 | CV544078 | AAG CAA AAG CAA TTA GTG GC | ATAGCAAGGCAAACAT CAATG | 134 | 57°C/25 |
| AMP diaminase | TC1176 | CV543772 | GAC ACT TAT GCC GGT TAT T | GGC GAT CAC ACC ATG CGT TAC | 136 | 52°C/25 |
| Multidrug resistance protein 11 | TC2799 | EH791098 | CAG CAG TGT GAA ATT AAT GGC | GTT TTT GGG GGT GGA TTG CAT | 111 | 57°C/25 |
| Ubiquitin | TC29 | CV543388 | CCA TAA CTC TTG AGG TGG AGA G | CTT CCC AGT CAA GGT CTT GAC | 480 | 55°C/25 |