Figure 1.

Rescue of the homozygous pdx2 mutant by vitamin B6. A, An immature silique from a heterozygous PDX2/pdx2 plant, the arrows indicate albino pdx2 seeds. B, Immature albino seeds isolated from PDX2/pdx2 plants and cultured in the presence (right) and absence (left) of vitamin B6; pictures were taken 14 d after 16-h-day/8-h-night cycles with a light intensity of 35 μmol photons m⁻² s⁻¹; a single wild-type seed was included on the −B6 plate as a control (arrow). C, Samples similar to B in the presence of vitamin B6 but under a higher magnification to emphasize the heterogeneous growth between pdx2 teratomata. D and E, The development of rescued pdx2 plants 15 and 28 d, respectively, after transfer of teratomata to soil; vitamin B6 supplementation was maintained during this time. The insets in E show dissected siliques from a rescued pdx2 plant in which all seeds display an albino phenotype and are later aborted. F, The top panel shows the chlorophyll content of PDX2/pdx2 plants (black bars) and rescued pdx2 plants (gray bars) cultured under the same conditions; the bottom panel shows the vitamin B6 content of the same plants. The measurements were performed 28 d after discontinuing supplementation with vitamin B6.