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Formation of the chromophoric adduct as a function of the concentration of PDX1. PDX1.1 was added at the time intervals indicated by arrows (10 μm at each time point) to a preincubation mix of PDX2 (10 μm), Gln (10 mm), and R5P (0.3 mm) in 50 mm Tris-Cl, pH 8.0.
