Table I.

Steady-state kinetic constants of PDX2

Kinetic parameters were determined as a function of the Gln concentration by measuring the PDX2 glutaminase activity using a coupled assay (Raschle et al., 2005). In the case of Arabidopsis, 10 μm each of PDX1 and PDX2 were used.

Species	kcat	Km	kcat/Km
	min−1	mm	mm min−1
B. subtilisa	7.6	0.99	7.68
P. falciparumb	6.6	0.56	11.8
Arabidopsis (full length)	0.23 ± 0.01	1.92 ± 0.32	0.12
Arabidopsis (ΔC30)	0.13 ± 0.01	2.62 ± 0.35	0.05

^aTaken from Raschle et al. (2005).

^bTaken from Gengenbacher et al. (2006).