Table I.
Gene | Length of Homology for HR
|
Experiment(s) | Seeds (Callia) Used | Surviving Calli | Targeted Calli | Frequencyb | |
---|---|---|---|---|---|---|---|
5′ | 3′ | ||||||
Experiment 1 | 1,050 (95) | 236 | 6c | 2.5% | |||
Adh2 | 6.2 kb | 6.0 kb | Experiment 2 | 1,900 (109) | 232 | 3d | 1.3% |
Total | 2,950 (204) | 468 | 9 | 1.9% | |||
Waxye | 6.3 kb | 6.8 kb | Total | 2,160 (194) | 638 | 6 | 0.94% |
The numerals in parentheses indicate the fresh weight of calli (grams) to be used for transformation.
The targeting frequency represents targeted calli per surviving callus.
One of the six calli regenerated slowly.
One of the three calli was accidentally lost.
The Waxy targeting represents a total of six independent experiments added with approximately 360 seeds in each experiment, and only the 3′-junction fragment of waxy can be amplified (Terada et al., 2002).