Table I.
Summary of the transformation experiments for targeting the Adh2 gene and comparison with the previous experiments for the Waxy gene
| Gene | Length of Homology for HR
|
Experiment(s) | Seeds (Callia) Used | Surviving Calli | Targeted Calli | Frequencyb | |
|---|---|---|---|---|---|---|---|
| 5′ | 3′ | ||||||
| Experiment 1 | 1,050 (95) | 236 | 6c | 2.5% | |||
| Adh2 | 6.2 kb | 6.0 kb | Experiment 2 | 1,900 (109) | 232 | 3d | 1.3% |
| Total | 2,950 (204) | 468 | 9 | 1.9% | |||
| Waxye | 6.3 kb | 6.8 kb | Total | 2,160 (194) | 638 | 6 | 0.94% |
a
The numerals in parentheses indicate the fresh weight of calli (grams) to be used for transformation.
b
The targeting frequency represents targeted calli per surviving callus.
c
One of the six calli regenerated slowly.
d
One of the three calli was accidentally lost.
e
The Waxy targeting represents a total of six independent experiments added with approximately 360 seeds in each experiment, and only the 3′-junction fragment of waxy can be amplified (Terada et al., 2002).