Table I.

Fatty acid composition of PE and PS in the psd1 psd2 psd3 triple mutant

Lipids were isolated from leaves or flowers, separated by two-dimensional TLC from wild type or psd1 psd2-1 psd3-1, and quantified by GC of fatty acid methyl esters. Data are given in mol% and represent mean ± sd of three measurements. Very long chain fatty acids (22:0 and 24:0) were only detected in PS but were absent from PE and all other membrane lipids analyzed.

Fatty Acid	Leaves		Flowers
	Wild-Type Col-0	psd1 psd2 psd3	Wild-Type Col-0	psd1 psd2 psd3
PE
16:0	21.3 ± 2.6	21.8 ± 0.5	21.3 ± 0.8	19.7 ± 1.0
16:1	0.4 ± 0.2	0.1 ± 0.1	0.5 ± 0.4	0.4 ± 0.2
16:2	0.2 ± 0.1	0.2 ± 0.0	0.3 ± 0.2	0.5 ± 0.1
16:3	1.0 ± 0.4	0.8 ± 0.5	0.1 ± 0.2	0.3 ± 0.0
18:0	0.9 ± 0.8	1.4 ± 0.1	1.6 ± 0.5	1.4 ± 0.1
18:1	2.6 ± 0.5	2.5 ± 0.1	2.6 ± 1.1	2.5 ± 0.3
18:2	38.5 ± 1.0	38.5 ± 1.1	36.1 ± 0.9	35.0 ± 0.7
18:3	33.8 ± 3.3	33.0 ± 0.9	35.8 ± 2.9	38.7 ± 2.5
PS
16:0	13.9 ± 1.0	15.0 ± 2.1	16.1 ± 5.2	13.6 ± 1.2
16:1	1.4 ± 0.6	1.2 ± 0.4	0.6 ± 0.4	0.8 ± 0.1
16:2	0.9 ± 0.4	1.4 ± 1.1	0.9 ± 0.2	0.7 ± 0.1
16:3	0.6 ± 0.1	0.8 ± 0.1	0.6 ± 0.8	0.2 ± 0.2
18:0	4.5 ± 0.8	4.0 ± 0.1	4.5 ± 1.1	5.3 ± 0.7
18:1	2.7 ± 0.7	2.4 ± 0.3	2.3 ± 0.5	1.8 ± 0.2
18:2	27.6 ± 1.0	26.9 ± 1.4	27.8 ± 3.1	25.0 ± 0.2
18:3	32.4 ± 2.2	35.3 ± 2.5	38.5 ± 5.1	41.6 ± 3.7
22:0	3.8 ± 0.5	3.5 ± 0.4	2.2 ± 0.2	3.1 ± 1.0
24:0	7.2 ± 1.3	6.6 ± 1.1	1.3 ± 0.1	2.0 ± 0.4