Detailed restriction map of the icmO-lphA-icmMLKEG locus. Coding regions are indicated by bold arrows. The site of the Tn903dIIlacZ insertions (LELA strains) are indicated by flags, and the sites of the kanamycin insertions (GS strains) are indicated by circles for insertions and squares for deletion substitutions. The restriction enzymes are as follows: B, BamHI; Bb, BsaBI; Bt, BstEII; D, DraIII; EI, EcoRI; E, Eco47III; N, NheI; Nd, NdeI; Nr, NruI; P, PstI; Pf, PflMI; S, SmaI (only relevant sites are marked). In the lower part, plasmids used for complementation studies are shown. In the upper part, chromosomal changes in the icmO-lphA region are presented.