Figure 3.

Analysis of the salt responsiveness of selected genes by qRT-PCR. Relative transcript numbers of the Plantago genes PmPLT1, PmPLT2, PmSUC2, PmSPS1, and PmSDH1 were quantified by qRT-PCR as described in “Materials and Methods” (n = 3). Based on macroarray analyses (not shown), PmFBPA1 was selected as control that revealed almost identical expression levels in the vascular tissue and the mesophyll and that is not influenced by salt treatment. The presented PmFBPA1 values show the absolute numbers of the qRT-PCRs as calculated from PmTUA2 reference dilutions.