Figure 5.
Heat treatment, extraction with an organic phase, and separation into different molecular size fractions of G. margarita medium. The [Ca2+]cyt change-inducing activity of the G. margarita medium (control) was unmodified by autoclaving. After extraction with ethyl acetate, it almost equally distributed between the organic phase and the aqueous phase. After separation into > and <3-kD subfractions, it was mainly recovered in the <3-kD fraction. Data are the means ± sem of three experiments. Δ[Ca2+]cyt indicates the variation in Ca2+ concentration between the peak and the basal level.