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. 2007 Jun;144(2):961–975. doi: 10.1104/pp.107.097568

Figure 1.

Figure 1.

Promoter:GUS or in situ assays of apyrase expression in various tissues. A, Representative staining for APY1:GUS or APY2:GUS in the region close to the root-hypocotyl junction (arrow) and in the more apical region of the differentiation zone of the primary root. B and C, Promoter:GUS expression in the apical region of primary root, including the elongation zone (brackets). Bar = 50 μm in A to C. D, In situ hybridization of digoxigenin-labeled antisense APY1 in primary root (top), and APY2 in lateral root (middle). Control (bottom) shows the lack of staining in a lateral root when the reverse transcriptase is left out of the PCR step of the sample preparation. E, Representative staining for APY1:GUS or APY2:GUS in the cotyledon. Bar = 100 μm. F, Representative staining for APY1:GUS or APY2:GUS in the mature cauline leaf. G, APY2:GUS expression in young leaf trichomes and the upper region of stipules (inset). H to L, Representative promoter:GUS staining for either APY1 or APY2 at various stages of flower development. There is no expression in flowers younger than stage 8 (H, black arrows), but some stigma staining between stages 9 and 12 (H, red arrows, I). J and K, Staining in flowers at stages 13 to 15. L, Staining of pollen tubes growing through style. M, Staining in abscission zone of sepal at the end of stage 15.