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. Author manuscript; available in PMC: 2008 Mar 1.
Published in final edited form as: Toxicon. 2006 Oct 14;49(3):318–328. doi: 10.1016/j.toxicon.2006.10.001

Fig. 1.

Fig. 1

Purification of two peptides, κA-PIVE and κA-PIVF, from milked venom of Conus purpurascens. Only the relevant portions of chromatograms are shown. (A) Venom was fractionated by preparative HPLC using a 0-90% gradient of B90 buffer over 100 min (see Materials and Methods). The fraction indicated by the bracket was found to contain κA-PIVE and κA-PIVF on further purification. (B) Analytical HPLC chromatogram of the fraction identified in panel A using a 20-50% gradient of B60 buffer over 30 min. The peaks corresponding to each peptide are labeled. (C) κA-PIVE had partially co-eluted with another peptide and was further purified to homogeneity.