Table 2.
Gateway recombination cloning of 34 LUCA and ECHH genes
| No. | Protein | Yield with His7-tag | Fusion with | Yield |
| 157 | aspartokinase I; homoserine dehydrogenase I | 1 | MBP | 3 |
| 145 | acetolactate synthase III small subunit | 1 | MBP | 3 |
| 203 | thiogalactoside acetyltransferase | 1 | MBP | 2 |
| 200 | 4-hydroxy-2-ketovalerate aldolase | 1 | MBP | 2 |
| 258 | putative ATPase | 1 | MBP | 3 |
| 204 | anthranilate synthase component II | 1 | MBP | 2 |
| 155 | putative ATP-binding component of a transport system | 1 | MBP | 3 |
| 201 | flagellum-specific ATP synthase | 1 | MBP | 2 |
| 259 | fused enoyl-CoA hydratase and epimerase | 1 | MBP | 2 |
| 156 | FFh | 1 | MBP | 2 |
| 146 | 30S ribosomal subunit protein S11 | 1 | MBP | 2 |
| 147 | 30S ribosomal subunit protein S13 | 1 | MBP | 3 |
| 148 | 50S ribosomal subunit protein L14 | 1 | MBP | 3 |
| 149 | 50S ribosomal subunit protein L22 | 1 | MBP | 3 |
| 151 | 50S ribosomal subunit protein L23 | 1 | MBP | 2 |
| 152 | 30S ribosomal subunit protein S12 | 1 | MBP | 2 |
| 150 | 50S ribosomal subunit protein L2 | 1 | MBP | 3 |
| 154 | 50S ribosomal subunit protein L1 | 1 | MBP | 3 |
| 153 | biotin- [acetyl-CoA carboxylase] holoenzyme synthetase | 1 | MBP | 3 |
| 159 | RNA polymerase beta prime subunit | 1 | MBP | 2 |
| 143 | predicted acyltransferase with acyl-CoA N-acyltransferase | 1 | MBP | 3 |
| 144 | putative proteoglycan | 1 | MBP | 2 |
| 158 | endonuclease III | 1 | MBP | 2 |
| 161 | dihydroxyacid dehydratase | 1 | MBP | 3 |
| 202 | part of formate-dependent nitrite reductase complex | 1 | NusA | 2 |
| 206 | conserved protein, member of DEAD box family | 1 | NusA | 2 |
| 198 | 50S ribosomal subunit protein L5 | 0 | NusA | 2 |
| 205 | O-6-alkylguanine-DNA/cysteine-protein methyltransferase | 0 | NusA | 1 |
| 199 | acetylornithine delta-aminotransferase | 0 | NusA | 1 |
| 197 | inducible ATP-independent RNA helicase | 1 | GST | 2 |
| 160 | glutamate synthase large subunit | 1 | - | |
| 207 | carbamoyl-phosphate synthase large subunit | 1 | - | |
| 208 | ATP-binding transport protein (membrane protein) | 0 | - | |
| 209 | Mg2+ transport ATPase, P-type 1 (membrane protein) | 0 | - |
Yields of purified proteins were analyzed by SDS-PAGE before recombination (proteins fused to His7-tag) and after recombination (each target protein fused to MBP-His7, GST-His7, or NusA-His6, respectively). The fusion partner that most efficiently improved protein yield is shown. With regard to yield, proteins were attributed to one of four categories: 3 (high), 2 (moderate), 1 (low), 0 (not purified).