Figure 4. Accuracy of Prediction of Hotspots at Coverage Levels of 15%.

Several approaches were introduced in the past for the prediction of interface residues. We applied methods that rely on different features to the task of predicting hotspots (to which none of them was optimized). The hydrophobic moment method represented the approach that relies exclusively on local physicochemical factors. The evolutionary approach was represented by the ET method, which relies on conservation to identify functionally important residues. A knowledge-based tool we introduced in the past represented the sequence-only approach. Finally, ProMate, a method for predicting interaction sites from unbound structures, represented the structure-based approach.