Figure 3.

Methylation pattern at allelic PW71 HpaII site is not clonally maintained in T cell subclones. T cell clone NS1.3, which exhibits a totally methylated pattern at PW71, was subcloned at limiting dilution and cultured for an additional 22–23 generations. Genomic DNA isolated from the subclones (NS1.3A–D) was analyzed as in Fig. 1A. Two of the subclones (NS1.A and B) exhibited a methylation pattern similar to the original clone NS1.3, but the remaining two subclones (NS1.3C and D) demonstrated the presence of the 4.7-kb band observed in peripheral blood (NS1.PHA).