FIGURE 7.

Fluorescent images of bacterium/phage complexes. Fluorescently labeled λ-phage were bound to Ymel at 37°C (a) and at 4°C (b), but the observation was made at room temperature. The labeling was carried out using a saturating amount of λ-phage (moi ≈ 2000) and incubated over 40 min in the λ-buffer. The uniformity of fluorescence indicates how the phage (or LamB receptors) are distributed on the bacterial cell wall. These images indicate that receptor distribution becomes less uniform as T is reduced, and is suggestive that they form large patches.