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(A,B) Conformational changes involving the O3-C3 carbonyl bond between the closed (A) (E2GFP) and open (B) (E2GFP·Cl) form. Water molecule and chloride are rendered as red and green spheres, respectively. Map shown is 2Fo-Fc contoured at 2δ. (C,D) Schematic representation of the closed (C) and open (D) conformations of E2GFP shows conformational changes and hydrogen bonding pattern around the chromophore.
