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(A) Fluorescence quenching (Stern-Volmer plot) of E2GFP with different halogens. Data collected at 23°C and pH = 7.95 ± 0.05. Fit parameter kd = 22.6 ± 0.3, 42.9 ± .9, 74.3 ± 1.3, and 182.6 ± 2 for I−, Br−, F−, and Cl−, respectively. (B) Fluorescence quenching (Stern-Volmer plot) at increasing chloride concentration for the GFP variants: E0GFP (−/−), EGFP (S56T/−), E1GFP (−/T203Y), and E2GFP (S65T/T203Y).
