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Detection of sequence variations in high-melting domains in exon 30 (C to A) and exon 31 (G to A) of the COL3A1 gene, and exon 5B (A to C) of the COL2A1 gene. Previously not detected or poorly detected sequence variations (22) were detected here in samples 1, 3, and 5 by using 15% polyacrylamide gels and 40 W/8.5 hr. Samples 2, 4, and 6 are controls.
