(A) A Southern blot (BglII digestion) showing hm1-homologous restriction fragments in Pr (lane 1), K61 (lane 2), K44 (lane 3), wheat (lane 4), rice (lane 5), sorghum (lane 6), Tripsacum (lane 7), oat (lane 8), and two different accessions of Teosinte (lanes 9 and 10). The blot was probed with the full length Hm1-cDNA and washed under conditions of high stringency. (B) An overlay of autorads of a gel blot that was probed first with Hm1 and then, after stripping, with ABG-459, (the barley STS, GenBank accession no. L43997). DNA of Pr (odd-numbered lanes) and Pr1 (even-numbered lanes) was digested with BamHI (lanes 1 and 2), EcoRI (lanes 3 and 4), SstI (lanes 5 and 6), and EcoRV (lanes 7 and 8).