Figure 4.

Determination of the transcriptional status of the SNRPN and the lacZ promoters in transgenic larvae carrying the PWSPX fragment. (A) Schematic illustration of the central part of the P[UZP1] transgene. SNRPN exon 1 and the 5′ portion of the lacZ gene are depicted as black boxes, and the GAL4 binding sites (UAS) are depicted as a black and white box. Arrows indicate the direction of transcription. Gray lines designate the sequences amplified by PCR. (B) Result of reverse transcription-PCR from two different strains transgenic for P[UZP1] in the presence (+) or absence (−) of GAL4. PCR products were separated on an agarose gel stained with ethidium bromide. Lane 1 shows control PCR products from a plasmid template, and lane 6 shows the absence of reverse transcription-PCR products when reverse transcriptase was omitted.