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Uptake (a) and transfection (b) of polyplexes in time. COS-7 cells were incubated with pDMAEMA- or PEI-based polyplexes for 60 minutes at 4°C. Subsequently, the cells were incubated at 37°C and after different incubation times the internalized fluorescence (a) or the luciferase expression was determined (b). The open bars represent pDMAEMA- and the filled bars PEI-incubated cells. Results are means ± SD from two different experiments.
