Table 2.
Gene expression of efflux transporters during endotoxemia
| Gene symbol | Protein synonym | Assay IDs | Delta CT valuesa | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| LPS− (controls) | LPS+t = 3 h | LPS+t = 6 h | LPS+t = 12 h | LPS+t = 24 h | LPS+t = 48 h | LPS+ + amino t = 6 h | LPS+ + amino t = 12 h | |||
| Abca1 | Abca 1 | Rn00710172_m1 | 7.1 ± 0.31 | 5.1 ± 0.16 | 5.8 ± 0.22 | 7.3 ± 0.55 | 7.3 ± 0.35 | 6.8 ± 0.23 | 6.1 ± 0.23 | 8.0 ± 0.12 |
| Abca2 | Abca 2 | Rn00577821_m1 | 3.5 ± 0.40 | 3.5 ± 0.35 | 4.1 ± 0.15 | 4.5 ± 0.38 | 4.3 ± 0.35 | 3.6 ± 0.06 | 4.4 ± 0.38 | 4.4 ± 0.12 |
| Abca5 | Abca 5 | Rn00597824_m1 | 9.8 ± 0.06 | 9.8 ± 0.35 | 9.8 ± 0.10 | 9.5 ± 0.12 | 10.0 ± 0.23 | 10.2 ± 0.21 | 9.5 ± 0.25 | 9.7 ± 0.35 |
| Abcb1a | P_gp | Rn00591394_m1 | 8.4 ± 0.01 | Fig. 5a | ||||||
| Abcb1b | P_gp | Rn00561753_m1 | 10.8 ± 0.04 | Fig. 5b | ||||||
| Abcb4 | Abcb 4 | Rn00562185_m1 | 10.7 ± 0.62 | 12.3 ± 0.16 | 12.5 ± 0.21 | 12.8 ± 0.15 | 10.9 ± 0.15 | 7.3 ± 0.42 | 13.2 ± 0.46 | 12.4 ± 0.24 |
| Abcb6 | Abcb 6 | Rn00589801_m1 | 5.5 ± 0.35 | 5.9 ± 0.26 | 6.4 ± 0.25 | 6.5 ± 0.52 | 5.9 ± 0.38 | 5.4 ± 0.14 | 6.4 ± 0.25 | 6.2 ± 0.32 |
| Abcb9 | Abcb 9 | Rn00573757_m1 | 8.1 ± 0.20 | 8.5 ± 0.38 | 9.3 ± 0.29 | 8.8 ± 0.17 | 8.6 ± 0.10 | 8.7 ± 0.17 | 9.2 ± 0.42 | 8.9 ± 0.15 |
| Abcb11 | Bsep | Rn00582179_m1 | 10.5 ± 0.40 | 8.6 ± 0.37 | 10.3 ± 0.24 | 11.9 ± 0.21 | 8.7 ± 0.17 | 5.4 ± 0.49 | 9.9 ± 0.21 | 9.7 ± 0.21 |
| Abcc1 | Mrp1 | Rn00574093_m1 | 5.9 ± 0.25 | 6.2 ± 0.26 | 6.1 ± 0.25 | 6.1 ± 0.25 | 6.3 ± 0.21 | 5.9 ± 0.23 | 6.4 ± 0.40 | 6.2 ± 0.15 |
| Abcc2 | Mrp2 | Rn00563231_m1 | 6.7 ± 0.31 | Fig. 5c | ||||||
| Abcc3 | Mrp3 | Rn00589786_m1 | 8.4 ± 0.15 | 8.7 ± 0.12 | 8.8 ± 0.508 | 8.6 ± 0.06 | 8.0 ± 0.35 | 8.2 ± 0.12 | 8.8 ± 0.32 | 8.7 ± 0.23 |
| Abcc4 | Mrp4 | Rn01465699_q1 | 6.6 ± 0.06 | Fig. 5d | ||||||
| Abcc5 | Mrp5 | Rn00588341_m1 | 7.1 ± 0.01 | 7.9 ± 0.03 | 7.6 ± 0.15 | 6.4 ± 0.06 | 6.5 ± 0.50 | 6.5 ± 0.38 | 7.6 ± 0.45 | 6.4 ± 0.36 |
| Abcc6 | Mrp6 | Rn00578778_m1 | 7.0 ± 0.14 | 7.0 ± 0.01 | 8.4 ± 0.21 | 8.4 ± 0.12 | 7.8 ± 0.05 | 6.8 ± 0.35 | 8.6 ± 0.07 | 8.3 ± 0.14 |
| Abcc8 | Sur1 | Rn00564778_m1 | 11.5 ± 0.40 | 11.4 ± 0.21 | 11.4 ± 0.26 | 13.4 ± 0.15 | 14.8 ± 0.15 | 13.2 ± 0.25 | 11.7 ± 0.23 | 13.7 ± 0.31 |
| Abcc9 | Sur2 | Rn00564842_m1 | 6.8 ± 0.01 | 7.7 ± 0.26 | 8.4 ± 0.31 | 8.1 ± 0.15 | 7.2 ± 0.32 | 6.8 ± 0.40 | 8.0 ± 0.35 | 8.2 ± 0.32 |
| Abcg1 | Abcg1 | Rn00585262_m1 | 6.8 ± 0.15 | 7.8 ± 0.25 | 6.8 ± 0.25 | 7.1 ± 0.40 | 6.8 ± 0.31 | 6.7 ± 0.21 | 6.4 ± 0.31 | 7.3 ± 0.36 |
| Abcg2 | Abcg2 | Rn00710585_m1 | 4.1 ± 0.24 | 4.5 ± 0.07 | 4.6 ± 0.21 | 5.0 ± 0.06 | 4.5 ± 0.14 | 4.5 ± 0.07 | 4.9 ± 0.42 | 5.2 ± 0.07 |
| Abcg5 | Abcg5 | Rn00587092_m1 | 18.8 ± 0.29 | b | ||||||
aData of nine pooled samples, measured in triplicate, are expressed as mean ± SD
bLow expressed transport protein: CT value is not considered as valid