Figure 2.

CDR3 of eight V_HDJ_H rearrangements using the DIR2 gene segment obtained from minilocus transgenic TdT^−/− mice. The V_H gene segment 3′ end and part of DIR2 coding sequence are indicated. For each clone, homologies with V_H and DIR coding sequences are indicated by dashes. Double lines indicate nucleotides that can derive from either gene segment, suggesting recombination at sites of short homologies between gene segments. The J_H gene segment is indicated at the right of the figure. P and N nucleotides are indicated. The CDR3 length is indicated in parenthesis when the rearrangement is productive, whereas nonproductive rearrangements are indicated by a dash.