Figure 2.

CT adjuvant is not essential for IR peptide vaccine induction of CTL. Mucosal peptide-induced CTL lyse targets endogenously expressing HIV gp160 envelope protein. Two different cell lines were used as a target cells: (i) 15–12 cells (BALB/c 3T3 fibroblasts transfected with HIV-1IIIB gp160 and endogenously expressing HIV gp160) (•, Left). These were compared with 18 Neo BALB/c 3T3 fibroblasts transfected with Neo^R alone as a control (▵, Left). (ii) P815 targets were tested in the presence (▪) or absence (◊) of I10 peptide (1 μM). SEM of triplicate cultures were all <5% of the mean.