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. 1982 Jun;107(3):316–321.

Lactate dehydrogenase (LDH) isozymes of human atherosclerotic plaques.

A M Gown, E P Benditt
PMCID: PMC1916248  PMID: 7081386

Abstract

We have been searching for additional markers to explore differences between the smooth muscle cells of human atherosclerotic fibrous plaques and their putative cells of origin in an aortic media and intima. Lactate dehydrogenase (LDH) isozyme analysis was performed on samples of human fibrous plaques selected by gross and microscopic criteria, and significant shifts in M4/M2H2 LDH isozyme ratios were found, relative to the underlying media and adjacent intima specimens. These changes are in the same direction seen in neoplastic tissues in vitro and in vivo and are probably not secondary to positional factors, inflammatory changes, or degenerative changes. The significance of these findings in relation to the monoclonal hypothesis of atherosclerosis is discussed.

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Selected References

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  1. Balinsky D., Cayanis E., Geddes E. W., Bersohn I. Activities and isoenzyme patterns of some enzymes of glucose metabolism in human primary malignant hepatoma. Cancer Res. 1973 Feb;33(2):249–255. [PubMed] [Google Scholar]
  2. Benditt E. P., Benditt J. M. Evidence for a monoclonal origin of human atherosclerotic plaques. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1753–1756. doi: 10.1073/pnas.70.6.1753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Benditt E. P., Gown A. M. Atheroma: the artery wall and the environment. Int Rev Exp Pathol. 1980;21:55–118. [PubMed] [Google Scholar]
  4. Binette P., Pragay D., Rekate A. Reverisbility of the lactate dehydrogenase isozyme shift induced by low oxygen tension. Life Sci. 1977 Jun 1;20(11):1809–1813. doi: 10.1016/0024-3205(77)90215-6. [DOI] [PubMed] [Google Scholar]
  5. Caltrider N. C., Lehman J. M. Changes in lactate dehydrogenase enzyme pattern in Chinese hamster cells infected and transformed with Simian virus 40. Cancer Res. 1975 Aug;35(8):1944–1949. [PubMed] [Google Scholar]
  6. DAOUD A., JARMOLYCH J., ZUMBO A., FANI, FLORENTIN R. PREATHEROMA PHASE OF CORONARY ATHEROSCLEROSIS IN MAN. Exp Mol Pathol. 1964 Oct;90:475–484. doi: 10.1016/0014-4800(64)90028-0. [DOI] [PubMed] [Google Scholar]
  7. Farber E. The sequential analysis of liver cancer induction. Biochim Biophys Acta. 1980 May 6;605(2):149–166. doi: 10.1016/0304-419x(80)90002-5. [DOI] [PubMed] [Google Scholar]
  8. Gartler S. M. Patterns of cellular proliferation in normal and tumor cell populations. Am J Pathol. 1977 Mar;86(3):685–692. [PMC free article] [PubMed] [Google Scholar]
  9. HAUST M. D., MORE R. H., MOVAT H. Z. The role of smooth muscle cells in the fibrogenesis of arteriosclerosis. Am J Pathol. 1960 Oct;37:377–389. [PMC free article] [PubMed] [Google Scholar]
  10. Heughan C., Niinikoski J., Hunt T. K. Oxygen tensions in lesions of experimental atherosclerosis of rabbits. Atherosclerosis. 1973 May-Jun;17(3):361–367. doi: 10.1016/0021-9150(73)90027-0. [DOI] [PubMed] [Google Scholar]
  11. Latner A. L., Turner D. M., Way S. A. Enzyme and isoenzyme studies in preinvasive carcinoma of the cervix. Lancet. 1966 Oct 15;2(7468):814–816. doi: 10.1016/s0140-6736(66)92251-3. [DOI] [PubMed] [Google Scholar]
  12. Lindy S., Uitto J., Uito J., Garbarsch C., Helin P., Lorenzen I. The effect of chronic hypoxia on lactate dehydrogenase in rabbit arterial wall. Biochemical studies on normal and injured aortas. Atherosclerosis. 1974 Sep-Oct;20(2):295–301. doi: 10.1016/0021-9150(74)90014-8. [DOI] [PubMed] [Google Scholar]
  13. Lojda Z., Fric P. Lactic dehydrogenase isoenzymes in the aortic wall. J Atheroscler Res. 1966 May-Jun;6(3):264–272. doi: 10.1016/s0368-1319(66)80008-x. [DOI] [PubMed] [Google Scholar]
  14. Moss N. S., Benditt E. P. The ultrastructure of spontaneous and experimentally induced arterial lesions. II. The spontaneous plaque in the chicken. Lab Invest. 1970 Sep;23(3):231–245. [PubMed] [Google Scholar]
  15. Niinikoski J., Heughan C., Hunt T. K. Oxygen tensions in the aortic wall of normal rabbits. Atherosclerosis. 1973 May-Jun;17(3):353–359. doi: 10.1016/0021-9150(73)90026-9. [DOI] [PubMed] [Google Scholar]
  16. Owens G. K., Rabinovitch P. S., Schwartz S. M. Smooth muscle cell hypertrophy versus hyperplasia in hypertension. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7759–7763. doi: 10.1073/pnas.78.12.7759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pearson T. A., Dillman J. M., Solex K., Heptinstall R. H. Clonal markers in the study of the origin and growth of human atherosclerotic lesions. Circ Res. 1978 Jul;43(1):10–18. doi: 10.1161/01.res.43.1.10. [DOI] [PubMed] [Google Scholar]
  18. Pearson T. A., Dillman J., Williams K. J., Wolff J. A., Adams R., Solez K., Heptinstall R. H., Malmros H., Sternby N. Clonal characteristics of experimentally induced "atherosclerotic" lesions in the hybrid hare. Science. 1979 Dec 21;206(4425):1423–1425. doi: 10.1126/science.505016. [DOI] [PubMed] [Google Scholar]
  19. Plum J., Ringoir S. Lactate dehydrogenase isoenzyme pattern as a measure of cellular differentiation in lymphocytic cells. J Reticuloendothel Soc. 1977 Apr;21(4):225–230. [PubMed] [Google Scholar]
  20. Poole J. C., Cromwell S. B., Benditt E. P. Behavior of smooth muscle cells and formation of extracellular structures in the reaction of arterial walls to injury. Am J Pathol. 1971 Mar;62(3):391–414. [PMC free article] [PubMed] [Google Scholar]
  21. Prasad R., Prasad N., Tevethia S. S. Expression of lactate and malate dehydrogenases in tumors induced by SV40 and 7,12-dimethylbenz(a)anthracene. Science. 1972 Oct 6;178(4056):70–71. doi: 10.1126/science.178.4056.70. [DOI] [PubMed] [Google Scholar]
  22. Ross R., Glomset J. A. The pathogenesis of atherosclerosis (second of two parts). N Engl J Med. 1976 Aug 19;295(8):420–425. doi: 10.1056/NEJM197608192950805. [DOI] [PubMed] [Google Scholar]
  23. Schaffner T., Taylor K., Bartucci E. J., Fischer-Dzoga K., Beeson J. H., Glagov S., Wissler R. W. Arterial foam cells with distinctive immunomorphologic and histochemical features of macrophages. Am J Pathol. 1980 Jul;100(1):57–80. [PMC free article] [PubMed] [Google Scholar]
  24. Schapira F. Isozymes and cancer. Adv Cancer Res. 1973;18:77–153. doi: 10.1016/s0065-230x(08)60751-4. [DOI] [PubMed] [Google Scholar]
  25. Setchenska M. S., Arnstein H. R. Changes in the lactate dehydrogenase isoenzyme pattern during differentiation of rabbit bone-marrow erythroid cells. Biochem J. 1978 Feb 15;170(2):193–201. doi: 10.1042/bj1700193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Stemerman M. B., Ross R. Experimental arteriosclerosis. I. Fibrous plaque formation in primates, an electron microscope study. J Exp Med. 1972 Oct 1;136(4):769–789. doi: 10.1084/jem.136.4.769. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Thomas W. A., Reiner J. M., Janakidevi K., Florentin R. A., Lee K. T. Population dynamics of arterial cells during atherogenesis. X. Study of monotypism in atherosclerotic lesions of black women heterozygous for glucose-6-phosphate dehydrogenase (G-6-PD). Exp Mol Pathol. 1979 Dec;31(3):367–386. doi: 10.1016/0014-4800(79)90038-8. [DOI] [PubMed] [Google Scholar]
  28. Wilens S. L., Malcolm J. A., Vazquez J. M. Experimental infarction (medial necrosis) of the dog's aorta. Am J Pathol. 1965 Oct;47(4):695–711. [PMC free article] [PubMed] [Google Scholar]
  29. Williams G. M. The pathogenesis of rat liver cancer caused by chemical carcinogens. Biochim Biophys Acta. 1980 May 6;605(2):167–189. doi: 10.1016/0304-419x(80)90003-7. [DOI] [PubMed] [Google Scholar]
  30. Woollams R., Barratt P. J., Orwell R. L., Piper D. W. LDH isoenzyme pattern of uninvolved gastric mucosa of patients with gastric carcinoma and benign gastric disease. Digestion. 1976;14(1):20–28. doi: 10.1159/000197796. [DOI] [PubMed] [Google Scholar]
  31. Zivković R. V., Djuricić B. M. Regional distribution of lactate dehydrogenase isoenzymes in adult rat brain. Experientia. 1975 Nov 15;31(11):1258–1260. doi: 10.1007/BF01945765. [DOI] [PubMed] [Google Scholar]

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