Table 1.
Development of Plasmodium berghei Δpplp5 parasites in Anopheles stephensi
| Exp. | Parasite | Oocysts |
Salivary gland sporozoites |
||||||
|---|---|---|---|---|---|---|---|---|---|
| n | Prevalence (%) | Mean | SEM | p-value | n | Mean | |||
| A | |||||||||
| 1 | wt | 52 | 88 | 221 | 22 | — | 20 | 4836 | |
| Δpplp5 clone 1 | 50 | 0 | 0 | 0 | p < 0.001 | 30 | 0 | ||
| 2 | wt | 50 | 96 | 111 | 20 | — | 30 | 3104 | |
| Δpplp5 clone 1 | 50 | 0 | 0 | 0 | p < 0.001 | 30 | 0 | ||
| Δpplp5 clone 2 | 50 | 0 | 0 | 0 | p < 0.001 | 30 | 0a | ||
| 3 | wt | 50 | 96 | 218 | 31 | — | 30 | 5166 | |
| Δpplp5 clone 1 | 50 | 0 | 0 | 0 | p < 0.001 | 30 | 0 | ||
| B | |||||||||
| 1 | wt | 50 | 100 | 249 | 17 | — | 15 | 9324 | |
| Δpplp5 clone 1 | 25 | 0 | 0 | 0 | p < 0.001 | 22 | 0 | ||
| Δpplp5 clone 2 | 50 | 4 | 0.04 | 0 | p < 0.001 | 13 | 0 | ||
| 2 | wt | 50 | 98 | 31 | 3 | — | n.d. | n.d. | |
| Δpplp5 clone 1 | 50 | 0 | 0 | 0 | p < 0.001 | n.d. | n.d. | ||
(A) Direct (gametocyte) feed on infected mice; (B) membrane feeding of in vitro cultivated ookinetes; Exp, experiment number; n, number of mosquitoes; Prevalence, percentage of mosquitoes with oocysts; Mean, mean number of oocysts or salivary gland sporozoites per mosquito, respectively; SEM, standard error of the mean; P-value as determined by z-test; n.d., not done; a1 single sporozoite observed.