Figure 2.

Ability of CD8⁺ cells to suppress HIV replication when separated from the infected macrophages by a transwell insert. Mφ were isolated from the PBMC of an HIV-seronegative donor, allowed to differentiate for 7 days and then infected with HIV-1_SF162. The infected Mφ were cultured in the absence or presence of activated CD8⁺ cells from an HIV-infected donor. The CD8⁺ cells were either cocultured directly with the infected Mφ or added to a transwell (0.45 μm filter) insert, which was placed over the infected Mφ. Cell-free culture fluids were collected every 3 days and monitored for particle-associated RT activity. The results were obtained during peak RT activity in the control group. The data are representative of two separate experiments giving similar results.