Table 1.
Effect of anti-RANTES, MIP-1α, and MIP-1β neutralizing antibodies on the ability of CD8+ cells to suppress HIV replication when separated from the infected Mφ by a transwell insert
| Exp. | GM-CSF | CD8+ cells* | Treatment | RANTES,† pg/ml | MIP-1α,† pg/ml | MIP-1β,† pg/ml | RT activity, ×10−3 cpm/ml |
|---|---|---|---|---|---|---|---|
| 1 | − | − | No antibody | <5 | 681 | 796 | 533.6 |
| − | +‡ | No antibody | <5 | 87.5 | 115.2 | 130.5 | |
| − | + | Control antibody§ | 247.1 | 269 | 220 | 158.1 | |
| − | + | anti-β-chemokine antibody¶ | <5 | <6 | 14.6 | 102.4 | |
| 2 | − | − | No antibody | <5 | 964.2 | 1453 | 729.1 |
| − | + | No antibody | 25.7 | 201 | 26.6 | 186.5 | |
| − | + | Control antibody | 26.5 | 571.7 | 714.8 | 72.7 | |
| − | + | anti-β-chemokine antibody | <5 | <6 | <4 | 152.8 | |
| 3 | + | − | No antibody | <5 | 180.4 | 27.1 | 1305.0 |
| + | + | No antibody | 126.9 | 190.4 | 114.1 | 267.1 | |
| + | + | anti-β-chemokine antibody | <5 | 6.4 | 6.1 | 99.0 |
*
Separated from macrophages by a transwell insert (0.45 μm pore size filter).
†
β-chemokine levels were determined by RANTES, MIP-1α, and MIP-1β specific ELISA.
‡
The level of RANTES, MIP-1α, and MIP-1β in the culture fluid of CD8+ cells in the absence of infected macrophages was 249.5, 24.9, and 20.3 pg/ml, respectively.
§
250 μg/ml of normal goat IgG.
¶
100 μg/ml of anti-RANTES, 50 μg/ml of anti-MIP-1α, and 100 μg/ml of anti-MIP-1β polyclonal goat antibodies.