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Northern blot analysis of PSCA expression. (A) Total RNA from normal prostate and LAPC-4 androgen-dependent (AD) and -independent (AI) prostate cancer xenografts were analyzed by using PSCA- or PSA-specific probes. Equivalent RNA loading and RNA integrity were demonstrated separately by ethidium staining for 18S and 28S RNA and are not shown. (B) Human multiple tissue Northern blot analysis of PSCA. The filter was obtained from CLONTECH and contains 2 μg of poly(A) RNA in each lane.
