Figure 3.

In situ hybridization with antisense RNA probe for human PSCA on normal and malignant prostate specimens. (A) PSCA is expressed by a subset of basal cells within the basal cell epithelium (black arrows) but not by the terminally differentiated secretory cells lining the prostatic ducts. (×400.) (B) PSCA is expressed strongly by a high-grade prostatic intraepithelial neoplasia (black arrow) and by invasive prostate cancer glands (yellow arrows) but is not detectable in normal epithelium (green arrow) at ×40 magnification. (C) Strong expression of PSCA in high-grade carcinoma. (×200.)