Figure 5.

In situ hybridization of biotin-labeled PSCA probes to human metaphase cells from phytohemagglutinin-stimulated peripheral blood lymphocytes. The chromosome 8 homologues are identified with arrows; specific labeling was observed at 8q24.2. (Inset) Partial karyotypes of two chromosome 8 homologues illustrating specific labeling at 8q24.2 (arrowheads). Images were obtained by using a Zeiss Axiophot microscope coupled to a cooled charge-coupled device camera. Separate images of 4′,6-diamidino-2-phenylindole-stained chromosomes and the hybridization signal were merged by using image analysis software (nu200 and image 1.57).