Figure 1.

Growth of parental and chimeric viruses in simian and mosquito cells. Chimeric virus inocula were grown in mosquito cells, parental LGT TP21 and E5 virus inocula were grown in simian Vero cells, whereas DEN4 parental virus inoculum for mosquito cells was grown in mosquito cells and DEN4 inoculum for simian cells was grown in simian Vero cells. Simian LLCMK₂ or Vero cells were infected with: (i) DEN4, TP21, or E5 virus at MOI of 0.01 or (ii) chimeric TP21/DEN4 or E5/DEN4 virus at MOI of 0.5. Mosquito C6/36 cells were infected with: (i) DEN4, TP21/DEN4, or E5/DEN4 virus at MOI of 0.01 or (ii) with TP21 or E5 virus at MOI of 1,000. Cells were harvested at indicated time (day after infection), and the virus titer was determined by a plaque assay on the same cells used for study of virus replication. Plaques were enumerated after 7 or 8 days of infection. In other experiments complete restriction of LGT TP21 and E5 in mosquito cells also was observed when MOI ranging from 0.01 to 1,000 were used.