Table 3.
Neurovirulence and neuroinvasiveness of parental and chimeric viruses in immunocompetent or SCID mice
| Virus | Neurovirulence for immunocompetent mice IC LD50 (PFU)
|
Neuroinvasiveness: i.p. LD50 (PFU)
|
||||
|---|---|---|---|---|---|---|
| Immunocompetent mice
|
SCID mice
|
|||||
| Unmodified virus | (preME)/DEN4 chimera | Unmodified virus | (preME)/DEN4 chimera | Unmodified virus | (preME)/DEN4 chimera | |
| TBEV* | 0.1 | 20 | 14 | >10,000,000 | Not tested | Not tested |
| LGT TP21 | 0.4 | 2,500 | 5,000 | >100,000 | 0.004 | >10,000,000† |
| LGT E5 | 20 | 110,000 | >10,000,000 | >100,000 | 0.060 | >10,000,000† |
| DEN4 | 8,000 | — | >10,000,000 | — | >10,000,000 | — |
*
Data from ref. 12 provided for purpose of comparison.
†
Virus was concentrated by centrifugation at 40,000 × g onto a 60% sucrose cushion.