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. 1998 Feb 17;95(4):1758–1763. doi: 10.1073/pnas.95.4.1758

Figure 4.

Figure 4

Antibodies to CyPA inhibit HIV-1 infection. (A). Sera from rabbits immunized with CyPA (anti-CyPA) or with AGE-modified CyPA (anti-AGE-CyPA) was used to reveal CyPA in a Western blot or immunoprecipitation (IP) assay. For Western analysis, a lysate of murine RAW 264.7 cells was fractionated on an SDS/PAGE. For IP analysis, lysate of RAW 264.7 cells metabolically labeled with [35S]methionine was immunoprecipitated with anti-CyPA or anti-AGE-CyPA serum (diluted 1:100) and fractionated on SDS/PAGE. (B) PBMCs were infected with HIV-1RF in the absence (control) or presence of affinity-purified anti-AGE-CyPA Ig. RT was measured in culture supernatants at days 6 and 8 after infection. Results are mean ± SE of three independent infections. (C) Primary human macrophages were infected with HIV-1ADA in the presence of anti-AGE-CyPA serum (1:40 dilution) or pre-immune (control) rabbit serum. Two hours after infection, cells were washed, treated with trypsin, and viral RNA was analyzed by RT-PCR by using pol-specific primers. Results of one out of two independent experiments are shown on the left, and both experiments are quantified on the Packard Direct Imager and plotted on the right as mean ± SE.