Figure 1.
Induction of tumors in nude mice by PTI-1. Pooled zeocin-resistant CREF-Trans 6 cells transfected with a pZeoSV vector or a 1.9-kb PTI-1 S cDNA, a 5′ UTR PTI-1 S DNA, or a Trun-EF PTI-1 S DNA cloned into a pZeoSV vector were injected at 1 × 106 cells per nude mouse (n = 4). Animals were also injected with untransfected CREF-Trans 6 cells, CREF-Trans 6:4 NMT cells (NMT; tumor-derived CREF-Trans 6 cells transfected with LNCaP high molecular weight DNA), and pooled CREF-Trans 6 cells transfected with the combination of a separate 5′ UTR PTI-1 S and a Trun-EF S DNA cloned into pZeoSV vectors. Tumors developed in the majority of 1.9-kb PTI-1 S cDNA and 4NMT injected animals by 7 days, and in all 1.9-kb PTI-1 S cDNA and 4NMT-injected animals by 10 days.