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Expression of PTI-1 in nude mouse tumor-derived CREF-Trans 6 cells. Tumors were isolated from animals and established in culture in the presence of zeocin, and total RNA was isolated and analyzed by Northern blotting. Northern blots were sequentially hybridized with a PTI-1, a zeocin-resistance, and then a GAPDH cDNA probe. NHPE, normal human prostate epithelial cells; PTI-1: NMT 1-1, 1-2, 2-2, 2-1, 3-1, 4-1, and 4-3 represent independent tumors isolated from different animals.
