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Inhibition of colony formation in PTI-1-expressing cells by antisense PT1–1. The indicated cell line was transfected with a pZeoSV plasmid containing or lacking an AS 0 to 500 PTI-1 5′ UTR, an AS Trun-EF region of PTI-1, or an AS PTI-1 1.9-kb sequence. Transfected cells were selected in zeocin and colony formation was determined. Results are the mean ± SD from four test plates per experimental condition. Similar results were obtained ±10% in two additional experiments.
