Figure 6.

Effect of antisense and random oligonucleotides on the proliferation of FRTL-5 cells. Note that the proliferative effect was reduced to 35% (∗, P < 0.05) when cells were cultured in medium containing 25 μM antisense oligonucleotides (bar antisense) compared with the proliferative effect of cells cultured in the presence or absence of 25 μM random oligonucleotides (bars control or random). The inhibitory effect of antisense oligonucleotides was overcome by the addition of 10 nM sAPP (bar antisense + sAPP). Note that increased concentrations of TSH (50 milliunits/ml) to FRTL-5 cells did not compensate for the inhibitory effect of 25 μM antisense oligonucleotides (bar antisense + TSH).