Figure 4.
Antibodies to chimeric VLPs inhibit VLP-mediated HA. A total of 60 ng of HPV16 L1/L2 VLPs were incubated for 1 h at ambient temperature with two-fold dilutions (1:400 to 1:3200, left to right) of prebleed serum or rabbit anti-VLP sera. Antisera were made either to native VLPs (N) or denatured VLPs (D). The samples were mixed with mouse erythrocytes at a final concentration of 0.5% (vol/vol) in 100 μl of PBS/0.1% BSA per well of a 96-well plate, incubated for 3 h at 4°C, and photographed.