Table 1.
The cDNA libraries used in the present investigation. The number of ESTs from each library that match genes in Arabidopsis thaliana, and the associated number of contigs and singletons are provided.
| Species and tissue or cell type | Relevant properties of tissue or cells | Name of library in NCBI and contributor(s) | Total number of ESTs per library (March 2006) | Number of ESTs matching genes in Arabidopsis (e < 10-7) | Number of contigs identified using CAP3 | Number of singletons identified using CAP3 | Unigenes (contigs plus singletons) |
|---|---|---|---|---|---|---|---|
| Zea mays | |||||||
| Sperm | Male | Zea mays sperm cell cDNA library (S. McCormick) Engel et al.[64] | 5174 | 2457 | 384 | 781 | 1165 |
| Egg | Female | Zea mays egg cell cDNA library (H. Yang and S. McCormick) Yang et al. [65] | 4085 | 1957 | 264 | 863 | 1127 |
| Triticum aestivum | |||||||
| Anther-meiotic | Male | Wheat meiotic anther cDNA library (O. Anderson) | 9139 | 6118 | 860 | 3058 | 3918 |
| Ovary | Female | Ov (Genoplante) | 5311 | 3699 | 813 | 1295 | 2108 |
| Brassica napus | |||||||
| Microspores (from 3 to 4 mm buds) | Male | 65JKBNM0 (M. Malik and J. Krochko) | 6539 | 5325 | 781 | 1620 | 2401 |
| Buds and young flowers | Heterogeneous (male, female and somatic) | ADIS-MPIZ 021 (B. Weisshaar) | 8747 | 6164 | 995 | 2665 | 3660 |