Table 3.

Mass isotopomer distributions of amino acids in glutamate production phase of C. glutamicum, where glutamate flux was 20 with respect to glucose uptake of 100.

		m0	m1	m2	m3	m4	m5
Ala-57	measured	0.702	0.129	0.047	0.122
	estimated	0.706	0.135	0.041	0.117
Gly-57	measured	0.782	0.087	0.131
	estimated	0.790	0.083	0.127
Val-57	measured	0.529	0.166	0.139	0.111	0.029	0.026
	estimated	0.527	0.178	0.148	0.111	0.019	0.017
Ser-57	measured	0.701	0.139	0.058	0.102
	estimated	0.708	0.139	0.058	0.095
Phe-57	measured	0.404	0.167	0.123	0.121	0.082	0.048
	estimated	0.381	0.181	0.135	0.135	0.088	0.041
Asp-57	measured	0.572	0.215	0.117	0.077	0.019
	estimated	0.575	0.211	0.130	0.075	0.008
Glu-57	measured	0.466	0.226	0.196	0.077	0.028	0.007
	estimated	0.462	0.236	0.202	0.073	0.021	0.005
His-57	measured	0.487	0.199	0.117	0.100	0.048	0.036
	estimated	0.492	0.228	0.112	0.082	0.035	0.045
Val-159	measured	0.550	0.164	0.210	0.050	0.026
	estimated	0.557	0.163	0.227	0.032	0.021
Leu-159	measured	0.452	0.231	0.198	0.083	0.028	0.008
	estimated	0.430	0.253	0.213	0.076	0.023	0.005
Ile-159	measured	0.471	0.225	0.194	0.076	0.026	0.008
	estimated	0.462	0.236	0.202	0.073	0.021	0.005
Ser-159	measured	0.721	0.158	0.121
	estimated	0.747	0.138	0.115
Phe-159	measured	0.418	0.169	0.167	0.092	0.077	0.037
	estimated	0.402	0.170	0.200	0.085	0.085	0.029

The underlined values are those used for ¹³C MFA based on the threshold (value > 0.06, error < 3%).-57 and -159 in amino acids indicate [M-57] and [M-159] groups of GC-MS analysis, whose ion fragments have high intensities [26, 42, 43].