Table 4.

Mass isotopomer distributions of amino acids in glutamate production phase of C. glutamicum, where glutamate flux was 68 with respect to glucose uptake of 100.

		m0	M1	m2	m3	m4	m5
Ala-57	measured	0.676	0.145	0.025	0.154
	estimated	0.670	0.140	0.042	0.148
Gly-57	measured	0.768	0.073	0.158
	estimated	0.769	0.072	0.159
Val-57	measured	0.463	0.189	0.164	0.133	0.021	0.030
	estimated	0.472	0.179	0.164	0.134	0.025	0.026
Ser-57	measured	0.651	0.170	0.082	0.097
	estimated	0.657	0.163	0.087	0.093
Phe-57	measured	0.324	0.160	0.150	0.150	0.104	0.051
	estimated	0.334	0.169	0.139	0.141	0.104	0.055
Asp-57	measured	0.586	0.185	0.102	0.116	0.010
	estimated	0.582	0.183	0.104	0.117	0.014
Glu-57	measured	0.435	0.211	0.227	0.091	0.028	0.009
	estimated	0.436	0.207	0.229	0.088	0.031	0.010
His-57	measured	0.477	0.223	0.099	0.073	0.032	0.080
	estimated	0.466	0.232	0.099	0.072	0.047	0.071
Val-159	measured	0.484	0.162	0.272	0.050	0.031
	estimated	0.495	0.168	0.263	0.042	0.031
Leu-159	measured	0.346	0.256	0.246	0.110	0.032	0.010
	estimated	0.359	0.258	0.237	0.103	0.034	0.009
Ile-159	measured	0.446	0.201	0.228	0.086	0.033	0.007
	estimated	0.436	0.207	0.229	0.088	0.031	0.010
Ser-159	measured	0.693	0.206	0.101
	estimated	0.689	0.203	0.108
Phe-159	measured	0.351	0.164	0.212	0.084	0.105	0.040
	estimated	0.350	0.162	0.214	0.084	0.105	0.037

The underlined values are those used for ¹³C MFA based on the threshold (value > 0.06, error < 3%).-57 and -159 in amino acids indicate [M-57] and [M-159] groups of GC-MS analysis, whose ion fragments have high intensities [26, 42, 43].