Table 1.
Effects of microemulsions on binding of OxRBCs and of apoptotic thymocytes by resident mouse peritoneal macrophages
Ligand | Addition | Concentration, μg/ml* | % inhibition |
---|---|---|---|
OxRBCs | LDL–lipid microemulsions (n = 6)† | 20–30 | 2 ± 3 |
OxLDL–lipid microemulsions (n = 7) | 20–30 | 87 ± 10 | |
PS liposomes (n = 8) | 30–35 | 95 ± 4 | |
Saturated-PS liposomes‡(n = 6) | 30–35 | 81 ± 8 | |
PC liposomes (n = 5) | 30–35 | 3 ± 4 | |
Oxidized SLPC liposomes§(n = 4) | 30–35 | 55 ± 10 | |
Apoptotic thymocytes | LDL–lipid microemulsion (n = 3) | 20–30 | 2 ± 2 |
OxLDL–lipid microemulsion (n = 2) | 20–30 | 47 ± 4 | |
PS liposomes (n = 4) | 30–35 | 89 ± 3 | |
Saturated-PS liposomes (n = 1) | 31 | 87 | |
PC liposomes (n = 1) | 33 | 0 | |
Oxidized SLPC liposomes (n = 1)§ | 33 | 33 |
Concentrations of liposome lipids added are expressed in terms of the total fatty acid content determined by gas liquid chromatography.
n indicates the number of experiments. Each experiment was done in triplicate.
Saturated PS liposomes were made from 1,2-dimyristoyl-phosphatidylserine or 1,2-dipalmitoyl-phosphatidylserine in a 1:1:1 molar ratio with unoxidized, fully saturated phosphatidylcholine and cholesterol.
1-stearoyl-2-linoleoyl-phosphatidylcholine (SLPC) that had been oxidized for 18–36 hr.