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. Author manuscript; available in PMC: 2008 Apr 30.

Published in final edited form as: Biochim Biophys Acta. 2007 Jan 25;1768(5):1238–1246. doi: 10.1016/j.bbamem.2007.01.012

Representative phase contrast photomicrographs (40x) of H&E stained 7 μm sections of electrically shock biceps femoris muscles are shown. Electroporation damage is distributed very non-uniformly in each muscle reflecting the stochastic nature of electroporation. The more shocks the more severe and the more uniform the damage. Longitudinal sections from muscle exposed to (a) 3 shock pulses exhibit a low percentage of damaged cells with mostly normal cytoskeletal architecture and some interstitial edema; (b) muscles subjected to 6 shocks manifest damage to 20–30% of the cells with more edema and (c) muscle subjected to 12 shocks have marked "contraction-band necrosis" to most cells and severe edema. Biopsies were taken 6 hours after electrical shock.

Representative phase contrast photomicrographs (40x) of H&E stained 7 μm sections of electrically shock biceps femoris muscles are shown. Electroporation damage is distributed very non-uniformly in each muscle reflecting the stochastic nature of electroporation. The more shocks the more severe and the more uniform the damage. Longitudinal sections from muscle exposed to (a) 3 shock pulses exhibit a low percentage of damaged cells with mostly normal cytoskeletal architecture and some interstitial edema; (b) muscles subjected to 6 shocks manifest damage to 20–30% of the cells with more edema and (c) muscle subjected to 12 shocks have marked "contraction-band necrosis" to most cells and severe edema. Biopsies were taken 6 hours after electrical shock.