Table 1. Primer sequences, annealing temperatures of primer sets (°C), and expected PCR fragment sizes (bp).
Primer Name | Primer sequence | Annealing temperature (°C) of primer set | cDNA PCR Product (bp) |
CCL25 qPCR | (S) GCCTACCACAGCCACATTAAG | 56 | 136 |
(AS) GCTTCCCGCACACCATCTT | |||
CCL28 qPCR | (S) GCTGCTGCACTGAGGTTTC | 56 | 145 |
(AS) TGAGGGCTGACACAGATTC | |||
CCR9 qPCR | (S) CCAGATGACTACGGCTATGAC | 56 | 150 |
(AS) GGCACCCACGATGAACAC | |||
CCR10 qPCR | (S) GCCCGCAGAGCAGGTTTCC | 60 | 136 |
(AS) CAAAGAGACACTGGGTTGGAAG | |||
GAPDH qPCR | (S) CTCAACGGGAAGCTCACTGG | 56 | 106 |
(AS) TGATCTCATCATACTTGGCAGGTT | |||
Foxp3 qPCR | (S) GGTGCAGTCTCTGGAACAAC | 63.3 | 148 |
(AS) GGTGCCAGTGGCTACAATAC | |||
IL-10 qPCR | (S) ACCAGATGGGCGACTTGTTG | 63 | 123 |
(AS) TCTCTGCCTTCGGCATTACG | |||
TGF-β qPCR | (S) GGTTCTGGATCAGGCTTACC | 57.1 | 101 |
(AS) CGCCAAACCTCTCCAAATCG | |||
Cdx-2 | (S) GGAACCTGTGCGAGTGGATG | 65 | 168 |
(AS) GCTCGGCCTTTCTCCGAATG | |||
GAPDH | (S) ACCACAGTCCATGCCATCAC | 60 | 452 |
(AS) TCCACCACCCTGTTGCTGTA |