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. 2007 Jul 25;2(7):e677. doi: 10.1371/journal.pone.0000677

Table 1. Primer sequences, annealing temperatures of primer sets (°C), and expected PCR fragment sizes (bp).

Primer Name Primer sequence Annealing temperature (°C) of primer set cDNA PCR Product (bp)
CCL25 qPCR (S) GCCTACCACAGCCACATTAAG 56 136
(AS) GCTTCCCGCACACCATCTT
CCL28 qPCR (S) GCTGCTGCACTGAGGTTTC 56 145
(AS) TGAGGGCTGACACAGATTC
CCR9 qPCR (S) CCAGATGACTACGGCTATGAC 56 150
(AS) GGCACCCACGATGAACAC
CCR10 qPCR (S) GCCCGCAGAGCAGGTTTCC 60 136
(AS) CAAAGAGACACTGGGTTGGAAG
GAPDH qPCR (S) CTCAACGGGAAGCTCACTGG 56 106
(AS) TGATCTCATCATACTTGGCAGGTT
Foxp3 qPCR (S) GGTGCAGTCTCTGGAACAAC 63.3 148
(AS) GGTGCCAGTGGCTACAATAC
IL-10 qPCR (S) ACCAGATGGGCGACTTGTTG 63 123
(AS) TCTCTGCCTTCGGCATTACG
TGF-β qPCR (S) GGTTCTGGATCAGGCTTACC 57.1 101
(AS) CGCCAAACCTCTCCAAATCG
Cdx-2 (S) GGAACCTGTGCGAGTGGATG 65 168
(AS) GCTCGGCCTTTCTCCGAATG
GAPDH (S) ACCACAGTCCATGCCATCAC 60 452
(AS) TCCACCACCCTGTTGCTGTA