Figure 1.

Target sequence cleavage of R1EN. (A) Substrate DNA used for oligonucleotide cleavage study. The substrate consists of the 40-bp long 28S rDNA sequence containing the target site of R1Bm. The sequence of target site duplication (TSD) is boxed. The X and Y indicate the original target sites on the bottom and top strands, respectively. One to seven represent cleaved sites other than the target sites (A and B). (B) Sequence-specific cleavage of both strands. Three picomoles of the substrates was incubated with 200 ng of H209A and R1EN at 25°C for 0, 30, 60 and 120 min and separated on a 30% polyacrylamide denaturing gel with sequencing markers. Cleavage sites are summarized in (A).