Table 3.
Kinetic parameters for folding (k1) and unfolding (k−1) of the quadruplex-forming oligonucleotides determined from analysis of the hysteresis between melting and annealing curves
| Sequence | k1 | k−1 | ||
|---|---|---|---|---|
| Ea (kJ mo1−1) | ln (A) s−1 | Ea (kJ mo1−1) | ln (A) s−1 | |
| G3T | - | - | - | - |
| G3T-T4-T | −93 ± 2 | −36 ± 1 | 182 ± 3 | 60 ± 1 |
| G3T4-T-T | −88 ± 3 | −34 ± 1 | 178 ± 2 | 58 ± 1 |
| G3T4-T-T4 | −48 ± 4 | −24 ± 1 | 173 ± 2 | 58 ± 1 |
| G3T4-T4-T | −59 ± 2 | −27 ± 1 | 174 ± 2 | 59 ± 1 |
| G3T4 | - | - | - | - |
The experiments were performed in 10 mM lithium phosphate pH 7.4 containing 20 mM potassium chloride. Ea is the activation energy (kJ.mol−1) and A is the pre-exponential factor from the equation k = Ae(–Ea/RT). No values are presented for G3T as it does not show any hysteresis, or G3T4 as more than one folded configuration exists in solution.