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. 2007 Jun 12;35(12):4164–4178. doi: 10.1093/nar/gkm387

Figure 2.

Figure 2.

Mutation of a G-rich sequence activates inclusion of PLP exon 3B. Results of RT-PCR assay of PLP and DM20 from total RNA isolated from Oli-neu cells (30 PCR cycles) (A) and L cells (35 PCR cycles) (B) transfected with wild-type PLP-neo (WT) and M1-MT to M10-MT. The PLP/DM20 ratios ± SD are shown (n = 3). GAPDH is used for accuracy of RNA loading (25 PCR cycles). Control represents the untransfected cells. The increase in PLP/DM20 ratio with the M2-MT construct is statistically significant (P = 0.017 for Oli-neu cells and P < 0.002 for L cells).

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