Figure 3.

Analysis of G triplets within M2 in Oli-neu cells and primary oligodendrocytes. (A) Partial wild-type sequences of exon 3B are shown and M2 is underlined. Linker scan mutations of M2 and G triplets within M2 are shown. (B) Results of RT-PCR assay of PLP and DM20 from RNA isolated from differentiated Oli-neu cells transfected with wild-type PLP-neo (WT) and mutated constructs, M2-MT, M2-MT2, G2-MT, G3-MT and G2-G3-MT. The PLP/DM20 ratios ± SD are shown (n = 3). The upper and lower limits of accurate quantification of the PLP/DM20 ratio are 10 and 0.01. (C) PLP and DM20 PCR products amplified in RNA extracted from differentiated OLs transfected with WT and M2-MT, M2-MT2 and G3-MT, duplicate transfections are shown.